h9n2 antigen  (Pulike Biological Engineering Inc)

Journal: Signal Transduction and Targeted Therapy

Article Title: Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin

doi: 10.1038/s41392-025-02227-6

Figure Lengend Snippet: Mutations in the GANC gene impair its ability to promote multiple influenza viruses replication. a – c Virus titers in supernatants from cells overexpressing GANC or its mutations, infected with H7N9 ( a ), H9N2 ( b ) or H1N1-PR8 ( c ) virus at a MOI of 0.1, were determined at 36 h post-infection using TCID 50 assays in MDCK cells. Western blotting analysis of HA protein levels in A549 cells transfected with plasmids encoding GANC or its mutations. The red pentagons indicate the locations of various mutations of GANC. Quantification was shown as mean ± SEM. n = 3 independent experiments. The Student’s t -test (unpaired, two-tailed) was used to compare two independent groups. *P < 0.05, ** P < 0.01. MOI multiplicity of infection, TCID 50 tissue culture infective dose

Article Snippet: Anti-H7N9 HA (40103-T62) and anti-H9N2 HA (11229-R106) were purchased from Sino Biological.

Techniques: Virus, Infection, Western Blot, Transfection, Two Tailed Test

Journal: Poultry Science

Article Title: A Novel PLGA-based nano-adjuvant augments mucosal and systemic immunity in chicks through intestinal targeting and IgA production

doi: 10.1016/j.psj.2026.106641

Figure Lengend Snippet: (A) Transmission electron microscopy (TEM) image of PEI-LSP-RA-PLGA, (B) Scanning electron microscopy (SEM) image of freeze-dried PEI-LSP-RA-PLGA, (C) Stability photographs, (D) Particle size change graph, (E) Zeta potential and PDI variation graph, (F) Loading efficiency of LSP, RA, and H9N2 in PEI-LSP-RA-PLGA, (G) In vitro release profile of H9N2.

Article Snippet: The H9N2 antigen (Luoyang Pulike, China) was mixed with the adjuvants PEI-LSP-RA-PLGA, PEI-RA-PLGA, LSP, and Alum (Thermo, USA) at a volume ratio of 1:3 and shaken on a shaker for 30 minutes to prepare PEI-LSP-RA-PLGA/H9N2, PEI-LSP-PLGA/H9N2, LSP/H9N2, and Alum/H9N2.

Techniques: Transmission Assay, Electron Microscopy, Zeta Potential Analyzer, In Vitro

Journal: Poultry Science

Article Title: A Novel PLGA-based nano-adjuvant augments mucosal and systemic immunity in chicks through intestinal targeting and IgA production

doi: 10.1016/j.psj.2026.106641

Figure Lengend Snippet: (A) Effects of different experimental groups on H9N2-IgG and H9N2-IgA content on chickens, (B) Effects of different experimental groups of IL-4, IL-5, IL-10, IL-12, and IFN-γ secretion on chickens. a-e Superscript different significant difference ( P < 0.05), n = 5.

Article Snippet: The H9N2 antigen (Luoyang Pulike, China) was mixed with the adjuvants PEI-LSP-RA-PLGA, PEI-RA-PLGA, LSP, and Alum (Thermo, USA) at a volume ratio of 1:3 and shaken on a shaker for 30 minutes to prepare PEI-LSP-RA-PLGA/H9N2, PEI-LSP-PLGA/H9N2, LSP/H9N2, and Alum/H9N2.

Techniques:

Journal: Scientific Reports

Article Title: Paper-based electrochemical immunosensor for label-free detection of multiple avian influenza virus antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes

doi: 10.1038/s41598-022-06101-1

Figure Lengend Snippet: Electrochemical immunosensors for detection of avian influenza viruses (H5N1, H7N9, and H9N2), their hemagglutinin (HA) proteins, or peptides derived from the hemagglutinin proteins.

Article Snippet: One mole of 2-(N-morpholino)ethanesulfonic acid (MES) buffer was obtained from Tech & Innovation (South Korea), dimethylformamide (DMF) was purchased from Daejung Chemicals & Metals Co., Ltd. (South Korea), and phosphate-buffered saline (1 × PBS, pH 7.4) was obtained from Thermo Fisher Scientific Inc. (USA); influenza A H5N1 HA polyclonal antibody (pAb) (Rabbit pAb, 11048-RP02), influenza A H7N9 HA pAb (Rabbit pAb, 40103-RP01), influenza A H9N2 HA pAb (Rabbit pAb, 11229-RP02), influenza A H5N1 HA proteins (A/Anhui/1/2005, 11048-V08H4), influenza A H7N9 HA proteins (A/Shanghai/1/2013, 40104-V08H), and influenza A H9N2 HA proteins (A/Hong Kong/35820/2009, 40174-V08B) were purchased from Sino Biological Inc. (China).

Techniques: Derivative Assay, Labeling, Fluorescence

Journal: Scientific Reports

Article Title: Paper-based electrochemical immunosensor for label-free detection of multiple avian influenza virus antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes

doi: 10.1038/s41598-022-06101-1

Figure Lengend Snippet: Differential pulse voltammograms of different concentrations of hemagglutinin antigens of the influenza virus (A) H5N1, (B) H7N9, and (C) H9N2 subtypes. (D) Calibration graphs of hemagglutinin antigens of influenza virus H5N1, H7N9, and H9N2 for the detection range from 100 pg/ml to 100 ng/ml. The error bars represent the standard deviations of three independent measurements.

Article Snippet: One mole of 2-(N-morpholino)ethanesulfonic acid (MES) buffer was obtained from Tech & Innovation (South Korea), dimethylformamide (DMF) was purchased from Daejung Chemicals & Metals Co., Ltd. (South Korea), and phosphate-buffered saline (1 × PBS, pH 7.4) was obtained from Thermo Fisher Scientific Inc. (USA); influenza A H5N1 HA polyclonal antibody (pAb) (Rabbit pAb, 11048-RP02), influenza A H7N9 HA pAb (Rabbit pAb, 40103-RP01), influenza A H9N2 HA pAb (Rabbit pAb, 11229-RP02), influenza A H5N1 HA proteins (A/Anhui/1/2005, 11048-V08H4), influenza A H7N9 HA proteins (A/Shanghai/1/2013, 40104-V08H), and influenza A H9N2 HA proteins (A/Hong Kong/35820/2009, 40174-V08B) were purchased from Sino Biological Inc. (China).

Techniques:

Journal: Scientific Reports

Article Title: Paper-based electrochemical immunosensor for label-free detection of multiple avian influenza virus antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes

doi: 10.1038/s41598-022-06101-1

Figure Lengend Snippet: (A) Differential pulse voltammograms and (B) peak currents of three BSA/Ab/COOH-CNT/CNT-PDMS electrodes of the proposed immunosensors after 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{\mu l}$$\end{document} μ l of 1 × PBS containing 10 ng/ml of H5N1 HA and 10 ng/ml of H9N2 HA was injected and incubated at room temperature for 30 min. (C) Selectivity of the immunosensors (H9N2-Ab functionalized electrodes) for influenza virus H9N2 hemagglutinin antigen detection. The test included 1 × PBS only, ten-fold diluted human serum only, non-targets (H5N1 HA, H1N1 virus, and MS2 bacteriophage) in 1 × PBS, and targets in 1 × PBS or ten-fold diluted human serum. (D) Reproducibility of the immunosensors (H5N1-Ab functionalized electrodes) using H5N1 HA antigen (concentration: 100 ng/ml). The error bars represent the standard deviations of three independent measurements.

Article Snippet: One mole of 2-(N-morpholino)ethanesulfonic acid (MES) buffer was obtained from Tech & Innovation (South Korea), dimethylformamide (DMF) was purchased from Daejung Chemicals & Metals Co., Ltd. (South Korea), and phosphate-buffered saline (1 × PBS, pH 7.4) was obtained from Thermo Fisher Scientific Inc. (USA); influenza A H5N1 HA polyclonal antibody (pAb) (Rabbit pAb, 11048-RP02), influenza A H7N9 HA pAb (Rabbit pAb, 40103-RP01), influenza A H9N2 HA pAb (Rabbit pAb, 11229-RP02), influenza A H5N1 HA proteins (A/Anhui/1/2005, 11048-V08H4), influenza A H7N9 HA proteins (A/Shanghai/1/2013, 40104-V08H), and influenza A H9N2 HA proteins (A/Hong Kong/35820/2009, 40174-V08B) were purchased from Sino Biological Inc. (China).

Techniques: Injection, Incubation, Concentration Assay